1A1

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A1Bacillus subtilis subsp. subtilistrpC2168
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium
Temperature: 37
Selected References: GenBank JADCUD000000000
Burkholder PR, Giles NH Jr. Induced Biochemical Mutations in Bacillus subtilis. American Journal of Botany. 1947 Jun;34(6):345-8. DOI: 10.1002/j.1537-2197.1947.tb12999.x. PMID: 20252518
Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983-95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678
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BGSC ← Dean DH ← Shapiro JA ← Sonenshein AL ← Schaeffer P ← Anagnostopoulos C

WGS of BGSC 1A1 is publicly available (GenBank: JADCUD000000000); compare with finished 4.22 Mb genome sequence from sequencing consortium (Barbe et al.; GenBank: AL009126). See also 1A866. Unable to swarm, form fruiting bodies, or form complex architecture in colonies on agar surfaces.

Other References

GenBank JADCUD000000000

Barbe V, Cruveiller S, Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Médigue C, Danchin A. From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology. 2009 Jun;155(Pt 6):1758-75. DOI: 10.1099/mic.0.027839-0. PMID: 19383706

Burkholder PR, Giles NH Jr. Induced Biochemical Mutations in Bacillus subtilisAmerican Journal of Botany. 1947 Jun;34(6):345-8. DOI: 10.1002/j.1537-2197.1947.tb12999.x. PMID: 20252518

Esbelin J, Malléa S, Clair G, Carlin F. Inactivation by Pulsed Light of Bacillus subtilis Spores with Impaired Protection Factors. Photochemistry and Photobiology. 2016 Mar;92(2):301-307. DOI: 10.1111/php.12568. PMID: 26790838

Hederstedt L, Magnusson K, Rutberg L. Reconstitution of succinate dehydrogenase in Bacillus subtilis by protoplast fusion. J Bacteriol. 1982 Oct;152(1):157-65. DOI: 10.1128/jb.152.1.157-165.1982. PMID: 6811547. PMCID: PMC221387

Kunst F, Ogasawara N, Moszer I, et al. The complete genome sequence of the Gram-positive bacterium Bacillus subtilisNature. 1997 Nov 20;390(6657):249-56. DOI: 10.1038/36786. PMID: 9384377

LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilisJournal of Bacteriology. 1986 Jan;165(1):204-214. DOI: 10.1128/jb.165.1.204-214.1986

Lampe MF, Bott KF. Cloning the gyrA gene of Bacillus subtilisNucleic Acids Research. 1984 Aug 10;12(15):6307-6323. DOI: 10.1093/nar/12.15.6307 PMID: 6089110, PMCID: PMC320074
 
McCarthy C, Nester EW. Heat-sensitive step in deoxyribonucleic acid-mediated transformation of Bacillus subtilisJournal of Bacteriology. 1969 Jan;97(1):162-165. DOI: 10.1128/jb.97.1.162-165.1969 PMID: 4974386, PMCID: PMC249569
 
McCarthy C, Nester EW. Macromolecular Synthesis in Newly Transformed Cells of Bacillus subtilisJournal of Bacteriology. 1967 Jul;94(1):131-140. DOI: 10.1128/jb.94.1.131-140.1967 PMID: 4961411, PMCID: PMC251881
 
Nilsson D, Hove-Jensen B. Phosphoribosylpyrophosphate synthetase of Bacillus subtilis. Cloning, characterization and chromosomal mapping of the prs gene. Gene. 1987;53(2-3):247-255. DOI: 10.1016/0378-1119(87)90013-8 PMID: 3038693
 
Saxild HH, Nygaard P. Gene-enzyme relationships of the purine biosynthetic pathway in Bacillus subtilisMolecular & General Genetics. 1988 Jan;211(1):160-167. DOI: 10.1007/BF00338408 PMID: 3125411
 
Sherwin SA, Carlton BC. Suppression of Tryptophan Mutants in Bacillus subtilis by Apparent Nonsense Suppressors. Genetics. 1971 Oct;69(2):133–143. DOI: 10.1093/genetics/69.2.133 PMID: 5002747, PMCID: PMC1212693
 
Shiwa Y, Matsumoto T, Yoshikawa H. Identification of laboratory-specific variations of Bacillus subtilis strains used in Japan. Bioscience, Biotechnology, and Biochemistry. 2013;77(10):2073-2076. DOI: 10.1271/bbb.130438
 
Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983-95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678