1A1

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A1Bacillus subtilis subsp. subtilistrpC2168
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium
Temperature: 37
References: GenBank JADCUD000000000
Barbe V, Cruveiller S, Kunst F, Lenoble P, Meurice G, Sekowska A, Vallenet D, Wang T, Moszer I, Médigue C, Danchin A. From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology. 2009 Jun;155(Pt 6):1758-75. DOI: 10.1099/mic.0.027839-0. PMID: 19383706
Burkholder PR, Giles NH Jr. Induced Biochemical Mutations in Bacillus subtilis. American Journal of Botany. 1947 Jun;34(6):345-8. DOI: 10.1002/j.1537-2197.1947.tb12999.x. PMID: 20252518
Hederstedt L, Magnusson K, Rutberg L. Reconstitution of succinate dehydrogenase in Bacillus subtilis by protoplast fusion. J Bacteriol. 1982 Oct;152(1):157-65. DOI: 10.1128/jb.152.1.157-165.1982. PMID: 6811547. PMCID: PMC221387
Kunst F, Ogasawara N, Moszer I, et al. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. DOI: 10.1038/36786. PMID: 9384377
Shiwa Y, Matsumoto T, Yoshikawa H. Identification of laboratory-specific variations of Bacillus subtilis strains used in Japan. Bioscience, Biotechnology, and Biochemistry. 2013;77(10):2073-2076. DOI: 10.1271/bbb.130438
Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983-95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678
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BGSC ← Dean DH ← Shapiro JA ← Sonenshein AL ← Schaeffer P ← Anagnostopoulos C

WGS of BGSC 1A1 is publicly available (GenBank: JADCUD000000000); compare with finished 4.22 Mb genome sequence from sequencing consortium (Barbe et al.; GenBank: AL009126). See also 1A866. Unable to swarm, form fruiting bodies, or form complex architecture in colonies on agar surfaces.