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1A1517

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1517	Bacillus subtilis subsp. subtilis	Δ(rapI-phrI)::{tet(M) cat} alrA::{(Psweet-rapI)spc} trpC2 pheA1 Cm Sp Tc	JAB773
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required in all media.
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

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