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1A1527

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1527	Bacillus subtilis subsp. subtilis	ΔattR::tet(M) ydhU::aphA-3 amyE::{(Pspank-rapI)spc} trpC2 pheA1 Km Sp Tc	JAB369
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium with kanamycin (5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml)
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

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