1A1608

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A1608Bacillus subtilissubsp. subtilisamyE::Phy-spank-ccaR PcpcG2-veg-sfgfp SpCcaSR TOM v0.2
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium containing spectinomycin (100 µg/ml)
Temperature: 37
References: Castillo SM, Baerman EA, Fujita M, Igoshin OA, Tabor JJ. Nat Commun. 2019 Jul 15;10(1):3099 (PMID: 31308373)
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Strain donated by Rice University. An MTA from Rice University must be fully executed prior to order fulfillment. Please email mta@rice.edu and copy ASC-microbioservice@osu.edu to initiate the MTA process.

Notes: Constructed in PY79 (= BGSC 1A747) genetic background; Transcriptional Output Module v0.2: IPTG-inducible expression of response regulator CcaR, sfGFP under the light-inducible promoter PcpcG2-veg