1A1609

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1608	Bacillus subtilissubsp. subtilis	thrC::PrpsD-hol-pcyA; xylA::Pxy!A(+47)-ccaSm3; amyE::Phy-spank-ccaR PcpcG2-veg-sfgfp Cm Em Sp	CcaSR vl.0
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium containing chloramphenicol (5 µg/ml), erythromycin (1 µg/ml), and spectinomycin (100 µg/ml)
Temperature: 37
References: Castillo SM, Baerman EA, Fujita M, Igoshin OA, Tabor JJ. Nat Commun. 2019 Jul 15;10(1):3099 (PMID: 31308373)

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Strain donated by Rice University. An MTA from Rice University must be fully executed prior to order fulfillment. Please email mta@rice.edu and copy ASC-microbioservice@osu.edu to initiate the MTA process.

Notes: Constructed in PY79 ( = BGSC lA 747) genetic background; Complete Ii ght-inducible two-component system CcaSR expressing sfGFP;