Picture of 1A1610

1A1610

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A1610Bacillus subtilissubsp. subtilisamyE::Phy-spank-t7rnap PT7lac-cph1(Y176H} ZcLacI-T7-cphl(Y176H)
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium containing zeocin (20 µg/ml)
Temperature: 37
References: Castillo SM, Baerman EA, Fujita M, Igoshin OA, Tabor JJ. Nat Commun. 2019 Jul 15;10(1):3099 (PMID: 31308373)
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Strain donated by Rice University. An MTA from Rice University must be fully executed prior to order fulfillment. Please email mta@rice.edu and copy ASC-microbioservice@osu.edu to initiate the MTA process.

Notes: Constructed in PY79 ( = BGSC 1A747) genetic background; IPTG-inducible expression of the PCB-dependent red fluorescent proteinI Cphl(Y176H)