1A1644

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A1644Bacillus subtilis subsp. subtilisPY79 ΔbsrA::spc (Spr)
ΔbsrB::kan (Kmr)
rpoCΩ pYQ52 (Cmr)
SG7
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with 10€¯Î¼g/mL kanamycin, 100€¯Î¼g/mL spectinomycin and 2.5€¯Î¼g/mL chloramphenicol
Temperature: 37
References: Sweetha Ganapathy, Jana Christin Wiegard, Roland K. Hartmann, Rapid preparation of 6S RNA-free B. subtilis σA-RNA polymerase and σA, Journal of Microbiological Methods, Volume 190, 2021, 106324, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2021.106324.
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