1A2

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A2Bacillus subtilis subsp. subtilisprototrophic
attSPβ		SB491
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium
Temperature: 37
References: GenBank EF191542
Whitt DD, Carlton BC. Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilis. J Bacteriol. 1968 Oct;96(4):1273–80. DOI: 10.1128/jb.96.4.1273-1280.1968. PMID: 4971887. PMCID: PMC252444
Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983–95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678
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Picture of 1A2

Other Designations: SB491

BGSC ← Lederberg J ← Shafer M

Prototrophic; contains an “island” of sequences derived from W23 in the trpC region; see GenBank accession EF191542. Cured of the SPβ prophage.