1A2

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A2Bacillus subtilis subsp. subtilisprototrophic
attSPβ
SB491
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium
Temperature: 37
Selected References: GenBank EF191542
Whitt DD, Carlton BC. Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilis. J Bacteriol. 1968 Oct;96(4):1273–80. DOI: 10.1128/jb.96.4.1273-1280.1968. PMID: 4971887. PMCID: PMC252444
Sherwin SA, Carlton BC. Suppression of Tryptophan Mutants in Bacillus subtilis by Apparent Nonsense Suppressors. Genetics. 1971 Oct;69(2):133–143. DOI: 10.1093/genetics/69.2.133 PMID: 5002747, PMCID: PMC1212693
Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983–95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678
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Other Designations: SB491

BGSC ← Lederberg J ← Shafer M

Prototrophic; contains an “island” of sequences derived from W23 in the trpC region; see GenBank accession EF191542. Cured of the SPβ prophage.

References: 

GenBank EF191542

Caudill ER, Tapia Hernandez R, Johnson KP, O'Rourke JT, Zhu L, Haynes CL, Feng ZV, Pedersen JA. Wall teichoic acids govern cationic gold nanoparticle interaction with Gram-positive bacterial cell walls. Chemical Science. 2020 Mar 23;11(16):4106–4118. DOI: 10.1039/c9sc05436g PMID: 34122876, PMCID: PMC8152635

Clement PL, Kuether JE, Borgatta JR, Buchman JT, Cahill MS, Qiu TA, Hamers RJ, Feng ZV, Haynes CL. Cobalt Release from a Nanoscale Multiphase Lithiated Cobalt Phosphate Dominates Interaction with Shewanella oneidensis MR-1 and Bacillus subtilis SB491. Chemical Research in Toxicology. 2020 Mar 16;33(3):806-816. DOI: 10.1021/acs.chemrestox.9b00465 PMID: 32013395

Gupta R, Elkabetz D, Leibman-Markus M, Sayas T, Schneider A, Jami E, Kleiman M, Bar M. Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues. The ISME Journal. 2022 Jan;16(1):122-137. DOI: 10.1038/s41396-021-01060-3 PMID: 34272494, PMCID: PMC8692462

McCarthy C, Nester EW. Heat-sensitive step in deoxyribonucleic acid-mediated transformation of Bacillus subtilisJournal of Bacteriology. 1969 Jan;97(1):162-165. DOI: 10.1128/jb.97.1.162-165.1969 PMID: 4974386, PMCID: PMC249569

Pramanik S, Hill SKE, Zhi B, Hudson-Smith NV, Wu JJ, White JN, McIntire EA, Kondeti VSSK, Lee AL, Bruggeman PJ, Kortshagen UR, Haynes CL. Comparative toxicity assessment of novel Si quantum dots and their traditional Cd-based counterparts using bacteria models Shewanella oneidensis and Bacillus subtilisEnvironmental Science: Nano. 2018;5(8):1890-1901. DOI: 10.1039/C8EN00332G

Qiu TA, Guidolin V, Hoang KN, Pho T, Carra' A, Villalta PW, He J, Yao X, Hamers RJ, Balbo S, Feng ZV, Haynes CL. Nanoscale battery cathode materials induce DNA damage in bacteria. Chemical Science. 2020 Sep 21;11(41):11244–11258. DOI: 10.1039/d0sc02987d PMID: 34094365, PMCID: PMC8162401

Qiu TA, Nguyen THT, Hudson-Smith NV, Clement PL, Forester D-C, Frew H, Hang MN, Murphy CJ, Hamers RJ, Feng ZV, Haynes CL. Growth-Based Bacterial Viability Assay for Interference-Free and High-Throughput Toxicity Screening of Nanomaterials. Analytical Chemistry. 2017 Mar;89(3):2057-2064. DOI: 10.1021/acs.analchem.6b04652 PMID: 28208291

Sherwin SA, Carlton BC. Suppression of Tryptophan Mutants in Bacillus subtilis by Apparent Nonsense Suppressors. Genetics. 1971 Oct;69(2):133–143. DOI: 10.1093/genetics/69.2.133 PMID: 5002747, PMCID: PMC1212693

Whitt DD, Carlton BC. Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilisJ Bacteriol. 1968 Oct;96(4):1273–80. DOI: 10.1128/jb.96.4.1273-1280.1968. PMID: 4971887. PMCID: PMC252444

Zawadzki P, Cohan FM. The size and continuity of DNA segments integrated in Bacillus transformation. Genetics. 1995 Dec;141(4):1231-1243. DOI: 10.1093/genetics/141.4.1231 PMID: 8601469, PMCID: PMC1206862

Zeigler DR, Prágai Z, Rodriguez S, Chevreux B, Muffler A, Albert T, Bai R, Wyss M, Perkins JB. The origins of 168, W23, and other Bacillus subtilis legacy strains. J Bacteriol. 2008 Nov;190(21):6983–95. DOI: 10.1128/JB.00722-08. PMID: 18723616. PMCID: PMC2580678