1A4

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A4Bacillus subtilis subsp. subtilispurE1
trpC2
alr-1
aroK906
KIT-2
QB928
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium with D-alanine (50 µg/ml)
Temperature: 37
Selected References: GenBank CP003783
Dedonder RA, Lepesant J, Lepesant-Kejzlarová J, Billault A, Steinmetz M, & Kunst F. (1977). Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Applied and Environmental Microbiology, 33(4), 989–993. DOI: 10.1128/aem.33.4.989-993.1977. PMCID: PMC170805
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Other Designation(s): QB928, KIT-2

BGSC ← Dedonder RA 

Donating Institution: Unité de Biochimie Cellulaire, Institut de Recherche en Biologie Moléculaire, Centre National de la Recherche Scientifique and Université Paris VII, 75221 Paris Cedex 05, France

Finished 4.15 Mb genome sequence available (GenBank CP003783); D-alanine is absolutely required in all media 

Other References: 

GenBank CP003783

Borriss R, Süss KH, Süss M, Manteuffel R, Hofemeister J. Mapping and properties of bgl (β-glucanase) mutants of Bacillus subtilisMicrobiology. 1986 Feb 1;132(2):431–42. DOI: 10.1099/00221287-132-2-431. PMID: 3842743

Dedonder RA, Lepesant J, Lepesant-Kejzlarová J, Billault A, Steinmetz M, & Kunst F. (1977). Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Applied and Environmental Microbiology, 33(4), 989–993. DOI: 10.1128/aem.33.4.989-993.1977. PMCID: PMC170805

Harford N. Genetic analysis of rec mutants of Bacillus subtilis. Evidence for at least six linkage groups. Mol Gen Genet. 1974 Mar 27;129(3):269–74. DOI: 10.1007/bf00267919. PMID: 4210037

Jarvis ED, Widom RL, LaFauci G, Setoguchi Y, Richter IR, Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilisGenetics. 1988 Nov;120(3):625-635. DOI: 10.1093/genetics/120.3.625 PMID: 2465199, PMCID: PMC1203540

LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilisJournal of Bacteriology. 1986 Jan;165(1):204-214. DOI: 10.1128/jb.165.1.204-214.1986

Lindgren V. Mapping of a genetic locus that affects glycerol 3-phosphate transport in Bacillus subtilisJournal of Bacteriology. 1978 Feb;133(2):667-670. DOI: 10.1128/jb.133.2.667-670.1978 PMID: 415047, PMCID: PMC222073

Loewen PC, Switala J. Genetic mapping of katA, a locus that affects catalase 1 level in Bacillus subtilisJournal of Bacteriology. 1987 Dec;169(12):5848-5851. DOI: 10.1128/jb.169.12.5848-5851.1987 PMID: 2824450, PMCID: PMC214185

Pai Y, Dabbs ER. Conditional lethal mutants of Bacillus subtilis dependent on kasugamycin for growth. Molecular & General Genetics. 1981;183(3):478-483. DOI: 10.1007/BF00268768 PMID: 6801425

Saxild HH, Nygaard P. Gene-enzyme relationships of the purine biosynthetic pathway in Bacillus subtilisMolecular & General Genetics. 1988 Jan;211(1):160-167. DOI: 10.1007/BF00338408 PMID: 3125411

Sugino A, Bott KF. Bacillus subtilis deoxyribonucleic acid gyrase. Journal of Bacteriology. 1980 Mar;141(3):1331-1339. DOI: 10.1128/jb.141.3.1331-1339.1980 PMID: 6245067, PMCID: PMC293831

Wilson CR, Morgan AE. Chromosomal-DNA amplification in Bacillus subtilisJournal of Bacteriology. 1985 Aug;163(2):445-453. DOI: 10.1128/jb.163.2.445-453.1985 PMID: 2991188, PMCID: PMC219142

Yu CS, Yim KY, Tsui SK, Chan TF. Complete genome sequence of Bacillus subtilis strain QB928, a strain widely used in B. subtilis genetic studies. J Bacteriol. 2012 Nov;194(22):6308–9. DOI: 10.1128/jb.01533-12. PMID: 23105055. PMCID: PMC3486399