1A6

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1A6Bacillus subtilis subsp. subtilisilvA1
pyrD1
thyA1
thyB1
trpC2
KIT-4
QB943
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium with thymine or thymidine (50 µg/ml)
Temperature: 37
Selected References:
Dedonder RA, Lepesant J, Lepesant-Kejzlarová J, Billault A, Steinmetz M, Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–93. DOI: 10.1128/aem.33.4.989-993.1977. PMCID: PMC170805
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Other Designation(s): QB934, KIT-3

BGSC ← Dedonder RA 

Donating Institution: Unité de Biochimie Cellulaire, Institut de Recherche en Biologie Moléculaire, Centre National de la Recherche Scientifique and Université Paris VII, 75221 Paris Cedex 05, France

Thymine/thymidine supplement is recommended for better growth, even in rich media

Other References: 

Borriss R, Süss KH, Süss M, Manteuffel R, Hofemeister J. Mapping and properties of bgl (β-glucanase) mutants of Bacillus subtilisMicrobiology. 1986 Feb 1;132(2):431–42. DOI: 10.1099/00221287-132-2-431. PMID: 3842743

Dedonder RA, Lepesant J, Lepesant-Kejzlarová J, Billault A, Steinmetz M, Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–93. DOI: 10.1128/aem.33.4.989-993.1977. PMCID: PMC170805

Donovan W, Zheng LB, Sandman K, Losick R. Genes encoding spore coat polypeptides from Bacillus subtilisJ Mol Biol. 1987 Jul 5;196(1):1–10. DOI: 10.1016/0022-2836(87)90506-7. PMID: 2821284

Jarvis ED, Widom RL, LaFauci G, Setoguchi Y, Richter IR, Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilisGenetics. 1988 Nov;120(3):625-635. DOI: 10.1093/genetics/120.3.625 PMID: 2465199, PMCID: PMC1203540

LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilisJournal of Bacteriology. 1986 Jan;165(1):204-214. DOI: 10.1128/jb.165.1.204-214.1986

Loewen PC, Switala J. Genetic mapping of katA, a locus that affects catalase 1 level in Bacillus subtilisJournal of Bacteriology. 1987 Dec;169(12):5848-5851. DOI: 10.1128/jb.169.12.5848-5851.1987 PMID: 2824450, PMCID: PMC214185

Miyakawa Y, Komano T. Study on the cell cycle of Bacillus subtilis using temperature-sensitive mutants. Molecular & General Genetics. 1981;181:207-214. DOI: 10.1007/BF00268428

Pai Y, Dabbs ER. Conditional lethal mutants of Bacillus subtilis dependent on kasugamycin for growth. Molecular & General Genetics. 1981;183(3):478-483. DOI: 10.1007/BF00268768 PMID: 6801425

Sugino A, Bott KF. Bacillus subtilis deoxyribonucleic acid gyrase. Journal of Bacteriology. 1980 Mar;141(3):1331-1339. DOI: 10.1128/jb.141.3.1331-1339.1980 PMID: 6245067, PMCID: PMC293831