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1P19

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1P19	Bacillusphage (No longer in available in collection)	Nf	Nf
Shipping Description: Lysate within screw-top vial
Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed.
Temperature: 37
References: Ofir G, Melamed S, Sberro H, Mukamel Z, Silverman S, Yaakov G, Doron S, Sorek R. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat Microbiol. 2018 Jan;3(1):90-98. doi: 10.1038/s41564-017-0051-0. Epub 2017 Oct 30. PMID: 29085076; PMCID: PMC5739279. Ofir G, Melamed S, Sberro H, Mukamel Z, Silverman S, Yaakov G, Doron S, Sorek R. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat Microbiol. 2018 Jan;3(1):90-98. doi: 10.1038/s41564-017-0051-0. Epub 2017 Oct 30. PMID: 29085076; PMCID: PMC5739279. Castilla-Llorente V, Salas M, Meijer WJ. Different responses to Spo0A-mediated suppression of the related Bacillus subtilis phages Nf and phi29. Environ Microbiol. 2009 May;11(5):1137-49. doi: 10.1111/j.1462-2920.2008.01845.x. Epub 2009 Jan 15. PMID: 19207565.

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