1P4

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1P4Bacillusphage (No longer in available in collection)SPO1SPO1
Shipping Description: Lysate within screw-top vial
Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed.
Temperature: 37
References: Ofir G, Melamed S, Sberro H, Mukamel Z, Silverman S, Yaakov G, Doron S, Sorek R. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat Microbiol. 2018 Jan;3(1):90-98. doi: 10.1038/s41564-017-0051-0. Epub 2017 Oct 30. PMID: 29085076; PMCID: PMC5739279.
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Other References: 

Mountain A, Baumberg S. Map locations of some mutations conferring resistance to arginine hydroxamate in Bacillus subtilis 168. Molecular & General Genetics. 1980;178(3):691–701. DOI: 10.1007/BF00337880 PMID: 6771491