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1P45

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1P45	Bacillusphage (No longer in available in collection)	phi29	phi29 Ï†29
Shipping Description: Lysate within screw-top vial
Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed.
Temperature: 37
References: Castilla-Llorente V, Salas M, Meijer WJ. Different responses to Spo0A-mediated suppression of the related Bacillus subtilis phages Nf and phi29. Environ Microbiol. 2009 May;11(5):1137-49. doi: 10.1111/j.1462-2920.2008.01845.x. Epub 2009 Jan 15. PMID: 19207565.

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