1S148

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
1S148Bacillus subtilis subsp. subtilisΔnprE
ΔaprE
Δepr
Δmpr
ΔnprB
Δvpr
Δbpr
ΔsigF
ΔwprA
ΔlacA::PxylA comK ermC		Δ8-22
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Thompson VC, McGuire BE, Frier MS, Legg MSG, Dyer TW, Gudavicius G, Potter S, Nano FE. Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes. FEMS Microbiol Lett. 2020 Oct 16;367(19):fnaa162. doi: 10.1093/femsle/fnaa162. PMID: 33016320.
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Picture of 1S148

Strain also has a resistance to chloramphenicol (50µg/mL) -June 2025

Strain shows significant instability and is not recommended for use. -May 2025

Suggested Alternatives: 1S149