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1S149

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1S149	Bacillus subtilis subsp. subtilis	ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr ∆wprA ΔsigF ΔlacA::PxylAcomK ermC Em	FN3
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium with erythromycin (1µg/ml) and lincomycin (25µg/ml)
Temperature: 37
References: Andrew M Rodd, Abrar Jawad, Francis E Nano, Bacillus subtilis small replicative plasmid collection incorporating five distinct origins of replication and compatible with Golden Gate DNA assembly, FEMS Microbiology Letters, Volume 370, 2023, fnad039, https://doi.org/10.1093/femsle/fnad039

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Strain also has a resistance to chloramphenicol (under 50µg/mL) -June 2025