BEC27960

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
BEC27960	Bacillus subtilis subsp. subtilis	trpC2 lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(rplU) Cm Em	BEC27960 CAG74760
Shipping Description: Nutrient agar #2 (Oxoid) with chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium containing chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Temperature: 37
References: Stork DA, Squyres GR, Kuru E, Gromek KA, Rittichier J, Jog A, Burton BM, Church GM, Garner EC, Kunjapur AM. Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights. bioRxiv 2021.02.19.432053; doi: https://doi.org/10.1101/2021.02.19.432053

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