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BGSC
BEC31630
BGSCID
Taxonomy Description
Gene Descriptor
Original Code
BEC31630
Bacillus subtilis subsp. subtilis
trpC2
lacA::Pxyl-dcas9
amyE::Pveg-sgRNA(mrpD)
Cm
Em
BEC31630
CAG74799
Shipping Description:
Nutrient agar #2 (Oxoid) with chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Growth Conditions:
LB, TBAB, TSA, Nutrient Agar, or other rich medium containing chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Temperature:
37
References:
Stork DA, Squyres GR, Kuru E, Gromek KA, Rittichier J, Jog A, Burton BM, Church GM, Garner EC, Kunjapur AM. Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights. bioRxiv 2021.02.19.432053; doi: https://doi.org/10.1101/2021.02.19.432053
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