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BGSC

Picture of 1A1514

1A1514

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1514	Bacillus subtilis subsp. subtilis	Δ(rapI-phrI)::{tet(M) cat} amyE::{(Pspank-rapI)spc} Cm Sp Tc	JAB842
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium with chloramphenicol (5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml)
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494. Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

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Picture of 1A1515

1A1515

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1515	Bacillus subtilis subsp. subtilis	Δ(ydcS-yddM)::{(PT7-GFPmut2) tet(M) cat (Pspank-T7RNAP)} thrC::{(int-yddJ) ?nicK mls} alrA::{(Psweet-rapI)spc} trpC2 pheA1 Cm Em Sp Tc	JAB1001
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

$0.00

Picture of 1A1516

1A1516

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1516	Bacillus subtilis subsp. subtilis	Δ(ydcS-yddM)::{(PT7-nifWLY78) (Pspank-T7RNAP) tet(M)} thrC::{(int-yddJ) ?nicK mls} amyE::{(Pspank-rapI)spc} alrA::cat trpC2 pheA1 Cm Em Sp Tc	JAB726
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

$0.00

Picture of 1A1517

1A1517

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1517	Bacillus subtilis subsp. subtilis	Δ(rapI-phrI)::{tet(M) cat} alrA::{(Psweet-rapI)spc} trpC2 pheA1 Cm Sp Tc	JAB773
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required in all media.
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

$0.00

Picture of 1A1518

1A1518

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1518	Bacillus subtilis subsp. subtilis	{Δ(ydcS-yddM) ?(xis-ydcL)}::{tet(M)cat} thrC::{(int-yddJ) ?nicK mls} alrA::{(Psweet-rapI) trpC2 pheA1 Cm Em Sp Tc	JAB854
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

$0.00

Picture of 1A1519

1A1519

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1519	Bacillus subtilis subsp. subtilis	{Δ(ydcS-yddM) ?(xis-ydcL)}::{yddF tet(M) cat} thrC::{(int-yddJ) ?nicK mls} alrA::{(Psweet-rapI)spc} trpC2 pheA1 Cm Em Sp Tc	JAB927
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium or other rich medium containing chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), spectinomycin (100 µg/ml), tetracycline (10 µg/ml), D-alanine (100 µg/ml). NOTE: D-alanine is absolutely required
Temperature: 37
References: Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. PMID: 30127494.

$0.00