BGSC
1A164
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A164 | Bacillus subtilis subsp. subtilis | sacR47 | QB642 |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium | |||
| Temperature: 37 | |||
| References: None found | |||
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1A1640
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A1640 | Bacillus subtilis subsp. subtilis | trpC pheA ICEBs10 (cured of ICEBs1), comK::spc(SpcR), str(StrR) | sJMP15 CAG80612 CAL89 |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB agar with spectinomycin (100 µg/ml) | |||
| Temperature: 37 | |||
| References: Peters, J.M., Koo, BM., Patino, R. et al. Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. Nat Microbiol 4, 244–250 (2019). https://doi.org/10.1038/s41564-018-0327-z | |||
$0.00
1A1641
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A1641 | Bacillus subtilis subsp. subtilis | trpC pheA ∆rapI-phrI::cat(CamR), amyE::Pspank-rapI+(SpcR) | sJMP251 CAG81072 |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium with chloramphenicol 6 µg/ml | |||
| Temperature: 37 | |||
| References: Peters, J.M., Koo, BM., Patino, R. et al. Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. Nat Microbiol 4, 244–250 (2019). https://doi.org/10.1038/s41564-018-0327-z | |||
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1A1643
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A1643 | Bacillus subtilis subsp. subtilis | PY79 ΔbsrA::spc (Spr) | MWΔbsrA |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB agar with spectinomycin (100 µg/ml) | |||
| Temperature: 37 | |||
| References: None found | |||
$0.00
1A1644
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A1644 | Bacillus subtilis subsp. subtilis | PY79 ΔbsrA::spc (Spr) ΔbsrB::kan (Kmr) rpoCΩ pYQ52 (Cmr) | SG7 |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with 10€¯Î¼g/mL kanamycin, 100€¯Î¼g/mL spectinomycin and 2.5€¯Î¼g/mL chloramphenicol | |||
| Temperature: 37 | |||
| References: Sweetha Ganapathy, Jana Christin Wiegard, Roland K. Hartmann, Rapid preparation of 6S RNA-free B. subtilis σA-RNA polymerase and σA, Journal of Microbiological Methods, Volume 190, 2021, 106324, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2021.106324. | |||
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1A1645
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1A1645 | Bacillus subtilis subsp. subtilis | trpC2 pheA1 polC-gfp mut2 (spcR) | KPL374 |
| Shipping Description: LB, TBAB, TSA, Nutrient Agar, or other rich medium containing spectinomycin (100 µg/ml) | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium containing spectinomycin (100 µg/ml) | |||
| Temperature: 30 | |||
| Selected References: Berkmen MB, Grossman AD. Spatial and temporal organization of the Bacillus subtilis replication cycle. Molecular Microbiology. 2006 Oct;62(1):57-71. DOI: 10.1111/j.1365-2958.2006.05356.x PMID: 16942601 | |||
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