BGSC
1E82
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E82 | Bacillus subtilis subsp. subtilis | ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr ΔsigF Km (pZY167::subC-v24) | V24 |
| Shipping Description: LB agar with kanamycin (5 µg/ml) | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium, or other rich medium containing kanamycin or neomycin (10 µg/ml) | |||
| Temperature: 37 | |||
| References: Thompson VC, McGuire BE, Frier MS, Legg MSG, Dyer TW, Gudavicius G, Potter S, Nano FE. Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes. FEMS Microbiol Lett. 2020 Oct 16;367(19):fnaa162. doi: 10.1093/femsle/fnaa162. PMID: 33016320. | |||
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1E83
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E83 | Bacillus subtilis subsp. subtilis | ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr ΔsigF Km (pZY167::subC-v25) | V25 |
| Shipping Description: LB agar with kanamycin (5 µg/ml) | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium, or other rich medium containing kanamycin or neomycin (10 µg/ml) | |||
| Temperature: 37 | |||
| References: Thompson VC, McGuire BE, Frier MS, Legg MSG, Dyer TW, Gudavicius G, Potter S, Nano FE. Temperature-sensitive recombinant subtilisin protease variants that efficiently degrade molecular biology enzymes. FEMS Microbiol Lett. 2020 Oct 16;367(19):fnaa162. doi: 10.1093/femsle/fnaa162. PMID: 33016320. | |||
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1E85
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E85 | Bacillus subtilis subsp. subtilis | REG19 Sp thrC::aadA PHS-K1E rnap; Ppen lacI | REG19/pBS4S::PHSK1 pol/Ppen::lacI |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium with Spectinomycin (50 µg/ml) | |||
| Temperature: 37 | |||
| References: Andrew M Rodd, Abrar Jawad, Francis E Nano, Bacillus subtilis small replicative plasmid collection incorporating five distinct origins of replication and compatible with Golden Gate DNA assembly, FEMS Microbiology Letters, Volume 370, 2023, fnad039, https://doi.org/10.1093/femsle/fnad039 | |||
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1E86
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E86 | Bacillus subtilis subsp. subtilis | (p110K) Km | |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB agar with kanamycin (30 µg/ml) | |||
| Temperature: 37 | |||
| References: Andrew M Rodd, Abrar Jawad, Francis E Nano, Bacillus subtilis small replicative plasmid collection incorporating five distinct origins of replication and compatible with Golden Gate DNA assembly, FEMS Microbiology Letters, Volume 370, 2023, fnad039, https://doi.org/10.1093/femsle/fnad039 | |||
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1E87
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E87 | Bacillus subtilis subsp. subtilis | (p110S) Sp | |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium with Spectinomycin (50 µg/ml) | |||
| Temperature: 37 | |||
| References: GenBank OP555912 GenBank OP555912 GenBank OP555912 GenBank OP555912 Andrew M Rodd, Abrar Jawad, Francis E Nano, Bacillus subtilis small replicative plasmid collection incorporating five distinct origins of replication and compatible with Golden Gate DNA assembly, FEMS Microbiology Letters, Volume 370, 2023, fnad039, https://doi.org/10.1093/femsle/fnad039 | |||
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1E88
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1E88 | Bacillus subtilis subsp. subtilis | (p110M) Li Em | |
| Shipping Description: Filter disks within foil packet | |||
| Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium with erythromycin (1 μg/ml) and lincomycin (25 μg/ml) | |||
| Temperature: 37 | |||
| References: GenBank OP555911 GenBank OP555911 GenBank OP555911 GenBank OP555911 Andrew M Rodd, Abrar Jawad, Francis E Nano, Bacillus subtilis small replicative plasmid collection incorporating five distinct origins of replication and compatible with Golden Gate DNA assembly, FEMS Microbiology Letters, Volume 370, 2023, fnad039, https://doi.org/10.1093/femsle/fnad039 | |||
$0.00