BGSC
1P19
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P19 | Bacillusphage (No longer in available in collection) | Nf | Nf |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed. | |||
| Temperature: 37 | |||
| References: Ofir G, Melamed S, Sberro H, Mukamel Z, Silverman S, Yaakov G, Doron S, Sorek R. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat Microbiol. 2018 Jan;3(1):90-98. doi: 10.1038/s41564-017-0051-0. Epub 2017 Oct 30. PMID: 29085076; PMCID: PMC5739279. Ofir G, Melamed S, Sberro H, Mukamel Z, Silverman S, Yaakov G, Doron S, Sorek R. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat Microbiol. 2018 Jan;3(1):90-98. doi: 10.1038/s41564-017-0051-0. Epub 2017 Oct 30. PMID: 29085076; PMCID: PMC5739279. Castilla-Llorente V, Salas M, Meijer WJ. Different responses to Spo0A-mediated suppression of the related Bacillus subtilis phages Nf and phi29. Environ Microbiol. 2009 May;11(5):1137-49. doi: 10.1111/j.1462-2920.2008.01845.x. Epub 2009 Jan 15. PMID: 19207565. | |||
$0.00
1P2
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P2 | Bacillusphage (No longer in available in collection) | PBS2 | PBS2 |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: Propagate on motile strain of B. subtilis | |||
| Temperature: 37 | |||
| References: None found | |||
$0.00
1P20
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P20 | Bacillusphage (No longer in available in collection) | susB47 | phi29 B47 |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed. | |||
| Temperature: 37 | |||
| References: None found | |||
$0.00
1P21
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P21 | Bacillusphage (No longer in available in collection) | susB610 | phi29 B610 |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed. | |||
| Temperature: 37 | |||
| References: None found | |||
$0.00
1P22
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P22 | Bacillusphage (No longer in available in collection) | SPP1v | SPP1v |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed. | |||
| Temperature: 37 | |||
| References: None found | |||
$0.00
1P25
| BGSCID | Taxonomy Description | Gene Descriptor | Original Code |
| 1P25 | Bacillusphage (No longer in available in collection) | Bace-11 | HER 414 |
| Shipping Description: Lysate within screw-top vial | |||
| Growth Conditions: To amplify, streak host confluently on LB agar and immediately drop ~10 µl of lysate on the lawn. Incubate at 30°C overnight. Cut out lysed area with surrounding bacteria and transfer to flask with 20 ml LB broth. Shake at 30°C until lysis observed. | |||
| Temperature: 30 | |||
| References: None found | |||
$0.00