BGSC

BEC41050

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BEC41050Bacillus subtilis subsp. subtilistrpC2
lacA::Pxyl-dcas9
amyE::Pveg-sgRNA(rnpA)
Cm
Em
BEC41050
CAG74840
Shipping Description: Nutrient agar #2 (Oxoid) with chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium containing chloramphenicol (5 µg/ml) and erythromycin (1 µg/ml)
Temperature: 37
References: Stork DA, Squyres GR, Kuru E, Gromek KA, Rittichier J, Jog A, Burton BM, Church GM, Garner EC, Kunjapur AM. Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights. bioRxiv 2021.02.19.432053; doi: https://doi.org/10.1101/2021.02.19.432053
$0.00

BKE00030

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BKE00030Bacillus subtilis subsp. subtilistrpC2
ΔyaaA::erm
BKE00030
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PMID: 28189581; PMCID: PMC5400513.
$0.00

BKE00040

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BKE00040Bacillus subtilis subsp. subtilistrpC2
ΔrecF::erm
BKE00040
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PMID: 28189581; PMCID: PMC5400513.
$0.00

BKE00050

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BKE00050Bacillus subtilis subsp. subtilistrpC2
ΔyaaB::erm
BKE00050
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PMID: 28189581; PMCID: PMC5400513.
$0.00

BKE00080

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BKE00080Bacillus subtilis subsp. subtilistrpC2
ΔyaaC::erm
BKE00080
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PMID: 28189581; PMCID: PMC5400513.
$0.00

BKE00100

BGSCIDTaxonomy DescriptionGene DescriptorOriginal Code
BKE00100Bacillus subtilis subsp. subtilistrpC2
ΔdacA::erm
BKE00100
Shipping Description: Nutrient agar #2 (Oxoid) with erythromycin (1 µg/ml)
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with erythromycin (1 µg/ml)
Temperature: 37
References: Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. PMID: 28189581; PMCID: PMC5400513.
$0.00