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BGSC

Picture of 1A1297

1A1297

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1297	Bacillus subtilis subsp. subtilis	trpC2 lacA::XylR_Pxyl-NarX_ErmR AmyE::SpcR_PydfJ-sfGFP_PlacI-NarL_Ppcn-LacI YdfHI::T7Pol_PT7-mCherry_CamR	iND77/iND27/iND138
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium supplemented with chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), and spectinomycin (60 µg/ml)
Temperature: 37
References: Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ. Phosphatase activity tunes two-component system sensor detection threshold. Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y. PMID: 29650958; PMCID: PMC5897336.

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Picture of 1A1298

1A1298

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1298	Bacillus subtilis subsp. subtilis	trpC2 YdfHI::CamR thrC::PlialG-NarL_KanR AmyE::SpcR_PydfJ-sfGFP_PlacI-NarX_Ppcn-LacI lacA::XylR_Pxyl-NarX(C415R)_ErmR	iND46/iND135/iND71/iND142
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium supplemented with chloramphenicol (5 µg/ml), erythromycin (0.5 µg/ml), kanamycin (5 µg/ml), and spectinomycin (60 µg/ml)
Temperature: 37
References: Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ. Phosphatase activity tunes two-component system sensor detection threshold. Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y. PMID: 29650958; PMCID: PMC5897336.

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Picture of 1A1299

1A1299

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1299	Bacillus subtilis subsp. subtilis	trpC2 Δskin Cm Δprophage1 Δprophage3 ΔSPbeta ΔPBSX pks::Cm ICEBs1(0)	DELTA6 Δ6
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium, or other rich medium containing chloramphenicol (5 µg/ml)
Temperature: 37
References: GenBank CP015975 Westers H, Dorenbos R, van Dijl JM, Kabel J, Flanagan T, Devine KM, Jude F, Seror SJ, Beekman AC, Darmon E, Eschevins C, de Jong A, Bron S, Kuipers OP, Albertini AM, Antelmann H, Hecker M, Zamboni N, Sauer U, Bruand C, Ehrlich DS, Alonso JC, Salas M, Quax WJ. Genome engineering reveals large dispensable regions in Bacillus subtilis. Mol Biol Evol. 2003 Dec;20(12):2076-90. doi: 10.1093/molbev/msg219. Epub 2003 Aug 29. PMID: 12949151.

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Picture of 1A13

1A13

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A13	Bacillus subtilis subsp. subtilis	car-41 leuC8 purF6 metA5	JAS-5 Mu8u5u6u41
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium
Temperature: 37
References: Kennett RH, Sueoka N. Regulation of the synthesis of DNA polymerase in Bacillus subtilis. J Mol Biol. 1971 Aug 28;60(1):31–44. DOI: 10.1016/0022-2836(71)90445-1. PMID: 4999102

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Picture of 1A130

1A130

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A130	Bacillus subtilis subsp. subtilis	aroA6	WB2281B
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, TSA, Nutrient Agar, or other rich medium
Temperature: 37
References: None found

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Picture of 1A1300

1A1300

BGSCID	Taxonomy Description	Gene Descriptor	Original Code
1A1300	Bacillus subtilis subsp. subtilis	dacA-mVenus-yfp Cm	PG2062
Shipping Description: Filter disks within foil packet
Growth Conditions: LB, TBAB, Nutrient Agar, or other rich medium supplemented with xylose (0.5% w/v) and chloramphenicol (5 µg/ml)
Temperature: 37
References: Lucena D, Mauri M, Schmidt F, Eckhardt B, Graumann PL. Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns. BMC Biol. 2018 Sep 3;16(1):97. doi: 10.1186/s12915-018-0561-0. PMID: 30173665; PMCID: PMC6120080.

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